Current Protocols Virtual Issue

Current Protocols is pleased to offer this issue of some of the most interesting cutting-edge articles in the areas of molecular and cell biology, from across all of the Current Protocols titles! Please explore our website and discover helpful features including tools and calculators, apps, videos, and webinars.

Current Protocols has been the leading source of laboratory protocols for over 25 years and comprises nearly 15,000 techniques and procedures across 17 titles. We have an extensive peer-review and editing process; our editorial boards are selective, and what we publish works. Current Protocols is a smart investment that will save time and money in the laboratory.  


Current Protocols features:

  • Meticulously detailed protocols for cutting-edge, classic, and basic methods, ensuring applicability towards all levels of research.
  • Alternate and supplemental methods for most protocols, empowering end-users to adapt techniques to suit their unique needs.
  • Access to the most modern techniques from authors at the best research institutions in the world, making you more competitive when publishing in primary literature.

Save time and valuable resources with Current Protocols.                       red-arrow Recommend to your librarian


Congratulations to 2012 Nobel Laureate Shinya Yamanaka!
Generation and Characterization of Human Induced Pluripotent Stem Cells
Mari Ohnuki, Kazutoshi Takahashi, Shinya Yamanaka
Full Text: PDF or HTML

Analysis of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae
Bessie W. Kebaara, Kristian E. Baker, Krista D. Patefield, Audrey L. Atkin
Full Text: PDF or HTML

Characterizing Synaptic Vesicle Proteins Using Synaptosomal Fractions and Cultured Hippocampal Neurons
Jerome DiGiovanni, Tao Sun, Zu‐Hang Sheng
Full Text: PDF or HTML

Derivation of Cerebellar Neurons from Human Pluripotent Stem Cells
Slaven Erceg, Dunja Lukovic, Victoria Moreno‐Manzano, Miodrag Stojkovic, Shomi S. Bhattacharya
Full Text: PDF or HTML

Design and Assembly of Large Synthetic DNA Constructs
Aleksandr E. Miklos, Randall A. Hughes, Andrew D. Ellington
Full Text: PDF or HTML

Determination of Sialylated and Neutral Oligosaccharides in Urine by Mass Spectrometry
Peter R. Clements
Full Text: PDF or HTML

DNA Origami: Synthesis and Self-Assembly
Arivazhagan Rajendran, Masayuki Endo, Hiroshi Sugiyama
Full Text: PDF or HTML

Engineering Designer Nucleases with Customized Cleavage Specificities
Jeffry D. Sander, Morgan L. Maeder, J. Keith Joung
Full Text: PDF or HTML

Generation of Spatio-Temporally Controlled Targeted Somatic Mutations in the Mouse
Daniel Metzger, Pierre Chambon
Full Text: PDF or HTML

Health Evaluation of Experimental Laboratory Mice
Tanya Burkholder, Charmaine Foltz, Eleanor Karlsson, C. Garry Linton, Joanne M. Smith
Full Text: PDF or HTML

High‐Efficiency Transfection and siRNA‐Mediated Gene Knockdown in Human Pluripotent Stem Cells
Yinghong Ma, Haifan Lin, Caihong Qiu
Full Text: PDF or HTML

High-Speed Multineuron Calcium Imaging Using Nipkow-Type Confocal Microscopy
Naoya Takahashi, Shigeyuki Oba, Naoto Yukinawa, Sakiko Ujita, Mika Mizunuma, Norio Matsuki, Shin Ishii, Yuji Ikegaya
Full Text: PDF or HTML

In-Gel Phosphatase Assay Using Fluorogenic and Radioactive Substrates
Isamu Kameshita
Full Text: PDF or HTML

Kinase-Catalyzed Biotinylation of Peptides, Proteins, and Lysates
Chamara Senevirathne, Keith D. Green, Mary Kay H. Pflum
Full Text: PDF or HTML

Laboratory Maintenance of Helicobacter Species
Thomas G. Blanchard, John G. Nedrud
Full Text: PDF or HTML

Measurement of Glucose Uptake in Cultured Cells
Norio Yamamoto, Manabu Ueda, Takuya Sato, Kengo Kawasaki, Keisuke Sawada, Kyuichi Kawabata, Hitoshi Ashida
Full Text: PDF or HTML

Measuring Altered Disposition of Xenobiotics in Experimental Models of Liver Disease
Rhiannon N. Hardwick, Nathan J. Cherrington
Full Text: PDF or HTML

Murine Embryonic Stem Cell Derivation, In Vitro Pluripotency Characterization, and In Vivo Teratoma Formation
Yu‐Fen Chou, Akiko Yabuuchi
Full Text: PDF or HTML

Oligonucleotide Microarrays for Clinical Diagnosis of Copy Number Variation and Zygosity Status
David T. Miller, Yiping Shen, Bai‐Lin Wu
Full Text: PDF or HTML

On-Bead Two-Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands
D. Gomika Udugamasooriya, Thomas Kodadek
Full Text: PDF or HTML

Orthotopic Models of Esophageal Carcinoma and Their Use in Drug Discovery
Stephanie J. Gros
Full Text: PDF or HTML

Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (
Josef Spidlen, Karin Breuer, Ryan Brinkman
Full Text: PDF or HTML

Survey of Protein Engineering Strategies
Uwe Bornscheuer, Romas J. Kazlauskas
Full Text: PDF or HTML

Using the Reactome Database
Robin Haw, Lincoln Stein
Full Text: PDF or HTML