Troubleshooting Tips

	Troubleshooting Tip 1: My cells look overstained. What should I change?
	Troubleshooting Tip 2: The transfection efficiency of my mammalian cell culture is very low. How can I improve this?
	Troubleshooting Tip 3: Nonspecific Staining is Observed. What Went Wrong?
	Troubleshooting Tip 4: The fluorescently-tagged version of my protein forms aggregates. What happened?
	Troubleshooting Tip 5: I engineered a fluorescently-tagged version of my protein but the fluorophore bleaches too rapidly during image acquisition. How can I fix this?
	Troubleshooting Tip 6: I want to assess cell viability, but do not know where to start.
	Troubleshooting Tip 7: My Western blotting doesn't work, although a neighboring lab used identical conditions successfully. How can I fix this?