Detection and Assay Methods

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Electroblotting from Polyacrylamide Gels
Aaron Goldman, Jeanine A. Ursitti, Jacek Mozdzanowski, David W. Speicher
Published online: November 2015
Digestion, Purification, and Enrichment of Protein Samples for Mass Spectrometry
Victoria E. Hedrick, Mercedes N. LaLand, Ernesto S. Nakayasu, Lake N. Paul
Published online: September 2015
Enzyme‐Linked Immunosorbent Assays
Peter V. Hornbeck
Published online: August 2015
Detergent Analysis in Protein Samples Using Mid‐Infrared (MIR) Spectroscopy
Chandreyee Das, Timothy Nadler, Ivona Strug
Published online: August 2015
Applications of Lipid Nanodiscs for the Study of Membrane Proteins by Surface Plasmon Resonance
Meg Trahey, Mavis Jiarong Li, Hyewon Kwon, Erica L. Woodahl, Wynton D. McClary, William M. Atkins
Published online: August 2015
Detection of Tyrosine Sulfation on Proteins
Yogita Kanan, Muayyad R. Al Ubaidi
Published online: April 2015
Diagnosing Lysosomal Storage Disorders: Mucopolysaccharidosis Type I
Britt A. Johnson, Angela Dajnoki, Olaf A. Bodamer
Published online: January 2015
Parallel Protein Detection by Solid‐Phase Proximity Ligation Assay with Real‐Time PCR or Sequencing
Tonge Ebai, Masood Kamali‐Moghaddam, Ulf Landegren
Published online: January 2015
Overview of Digital Electrophoresis Analysis
Butch Moomaw, Scott Medberry, Sean R. Gallagher
Published online: October 2014
Tetra Detector Analysis of Membrane Proteins
Larry J.W. Miercke, Rebecca A. Robbins, Robert M. Stroud
Published online: August 2014
General qPCR and Plate Reader Methods for Rapid Optimization of Membrane Protein Purification and Crystallization Using Thermostability Assays
Thomas M. Tomasiak, Bjørn P. Pedersen, Sarika Chaudhary, Andrew Rodriguez, Yaneth Robles Colmanares, Zygy Roe‐Zurz, Sobha Thamminana, Meseret Tessema, Robert M. Stroud
Published online: August 2014
Western Blot Assay for Quantitative and Qualitative Antigen Detection in Vaccine Development
Sanjai Kumar, Hong Zheng, Babita Mahajan, Yukiko Kozakai, Merribeth Morin, Emily Locke
Published online: May 2014
Penicillin‐Binding Protein Imaging Probes
Ozden Kocaoglu, Erin E. Carlson
Published online: December 2013
Diagnosing Lysosomal Storage Disorders: Mucopolysaccharidosis Type II
Britt A. Johnson, Otto P. van Diggelen, Angela Dajnoki, Olaf A. Bodamer
Published online: October 2013
Computational Large‐Scale Mapping of Protein‐Protein Interactions Using Structural Complexes
Benjamin Shoemaker, Stefan Wuchty, Anna R. Panchenko
Published online: September 2013
Overview of Current Methods in Sedimentation Velocity and Sedimentation Equilibrium Analytical Ultracentrifugation
Huaying Zhao, Chad A. Brautigam, Rodolfo Ghirlando, Peter Schuck
Published online: February 2013
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Colin D. White, Alex Toker
Published online: January 2013
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