Detection and Assay Methods

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Tetra Detector Analysis of Membrane Proteins
Larry J.W. Miercke, Rebecca A. Robbins, Robert M. Stroud
Published online: August 2014
General qPCR and Plate Reader Methods for Rapid Optimization of Membrane Protein Purification and Crystallization Using Thermostability Assays
Thomas M. Tomasiak, Bjørn P. Pedersen, Sarika Chaudhary, Andrew Rodriguez, Yaneth Robles Colmanares, Zygy Roe‐Zurz, Sobha Thamminana, Meseret Tessema, Robert M. Stroud
Published online: August 2014
Western Blot Assay for Quantitative and Qualitative Antigen Detection in Vaccine Development
Sanjai Kumar, Hong Zheng, Babita Mahajan, Yukiko Kozakai, Merribeth Morin, Emily Locke
Published online: May 2014
Penicillin‐Binding Protein Imaging Probes
Ozden Kocaoglu, Erin E. Carlson
Published online: December 2013
Diagnosing Lysosomal Storage Disorders: Mucopolysaccharidosis Type II
Britt A. Johnson, Otto P. van Diggelen, Angela Dajnoki, Olaf A. Bodamer
Published online: October 2013
Computational Large‐Scale Mapping of Protein‐Protein Interactions Using Structural Complexes
Benjamin Shoemaker, Stefan Wuchty, Anna R. Panchenko
Published online: September 2013
Overview of Current Methods in Sedimentation Velocity and Sedimentation Equilibrium Analytical Ultracentrifugation
Huaying Zhao, Chad A. Brautigam, Rodolfo Ghirlando, Peter Schuck
Published online: February 2013
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Colin D. White, Alex Toker
Published online: January 2013
Proteolytic Fingerprinting of Complex Biological Samples Using Combinatorial Libraries of Fluorogenic Probes
Kalyani Jambunathan, Douglas S. Watson, Krishna Kodukula, Amit K. Galande
Published online: November 2012
Metallomics Using Inductively Coupled Plasma Mass Spectrometry
Brian J. Vaccaro, Angeli L. Menon, W. Andrew Lancaster, Michael W. W. Adams
Published online: September 2012
Staining Proteins in Gels
Sean R. Gallagher, Joachim Sasse
Published online: August 2012
Analysis of Protein Turnover by Quantitative SNAP‐Based Pulse‐Chase Imaging
Dani L. Bodor, Mariluz Gómez Rodríguez, Nuno Moreno, Lars E.T. Jansen
Published online: June 2012
One‐Dimensional SDS Gel Electrophoresis of Proteins
Sean R. Gallagher
Published online: April 2012
HPLC‐SEC Characterization of Membrane Protein‐Detergent Complexes
Alla Korepanova, Edmund D. Matayoshi
Published online: April 2012
Single‐Cell Phospho‐Protein Analysis by Flow Cytometry
Kenneth R. Schulz, Erika A. Danna, Peter O. Krutzik, Garry P. Nolan
Published online: February 2012
Radioligand Binding Analysis as a Tool for Quality Control of GPCR Production for Structural Characterization: Adenosine A2aR as a Template for Study
Shweta Singh, Minghao Zhang, Nicolas Bertheleme, Elodie Kara, Philip G. Strange, Bernadette Byrne
Published online: February 2012
One‐Dimensional SDS Gel Electrophoresis of Proteins
Sean R. Gallagher
Published online: January 2012
Analysis of Protein Ubiquitination
Jeffrey D. Laney, Mark Hochstrasser
Published online: November 2011
Immunoblotting and Immunodetection
Sean Gallagher, Scott E. Winston (tank transfer systems), Steven A. Fuller (tank transfer systems), John G.R. Hurrell (tank transfer systems; reversible staining of proteins)
Published online: September 2011
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