This unit describes sample preparation and sectioning methods for frozen tissue. Sections of this type are used in a variety of light microscopic procedures including in situ hybridization immunohistochemistry, and enzyme histochemistry.
Basic Protocol 1: Specimen Preparation and Sectioning
Support Protocol 1: Tissue Fixation and Sucrose Infusion
Support Protocol 2: Fixation of Cryosections for In Situ Hybridization
Figure a0.3E.1 Sectioning procedure. A to E illustrate cryosectioning and correspond to steps to of ; C to E also illustrate paraffin wax sectioning and correspond to steps to of , APPENDIX.
|Hollands, B. 1962. Histochemistry and microtomy of fresh‐frozen tissue. In Progress in Medical Laboratory Technique (F.J. Baker, ed.) pp. 112‐135. Butterworth, London.|
|These references describe the basic cryosectioning method and applications in clinical pathology.|
|Zugibe, F. 1970. Diagnostic Histochemistry. C.V. Mosby, St. Louis.|