Dietary Manipulation of Mouse Metabolism

Jérôme N. Feige1, Marie Lagouge1, Johan Auwerx2

1 Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France, 2 Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 29B.5
DOI:  10.1002/0471142727.mb29b05s84
Online Posting Date:  October, 2008
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Abstract

The maintenance of metabolic homeostasis relies on the balanced intake of nutrients from food. Consequently, diet composition strongly impacts whole‐body physiology. Dietary formulations with strong nutrient imbalances can lead to metabolic disorders, with lipids and simple sugars playing a prominent role. This unit describes how diet formulation can be modified to generate mouse models of human metabolic pathologies, and it details methodological procedures linked to dietary manipulations, including caloric restriction and introduction of a test compound. Curr. Protoc. Mol. Biol. 84:29B.5.1‐29B.5.12. © 2008 by John Wiley & Sons, Inc.

Keywords: metabolism; diet; mouse; diabetes; obesity

     
 
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Table of Contents

  • Introduction
  • Basic Protocol 1: Induction of Metabolic Pathologies Via Dietary Manipulation
  • Basic Protocol 2: Administration of a Test Compound Through the Diet
  • Basic Protocol 3: Caloric Restriction
  • Commentary
  • Literature Cited
  • Figures
  • Tables
     
 
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Materials

Basic Protocol 1: Induction of Metabolic Pathologies Via Dietary Manipulation

  Materials
  • Appropriate mouse strain (e.g., C57BL/6 or see )
  • Mouse diet (standard or high‐fat diet) in powdered form
  • Test compound
  • Vehicle to dissolve compound (if required)
  • Group housing cages for mice
  • Scale with 0.1 g accuracy (to weigh food and mice)
  • Container to accommodate mice during weighing
  • Scale with 0.1 mg accuracy (to weigh compound)
  • Mortar and pestle for grinding the compound (if required)
  • Large container for mixing diet and test compound or automated mixing machine
  • Commercial pelleting machine (optional)
  • Desiccator (optional)

Basic Protocol 2: Administration of a Test Compound Through the Diet

  Materials
  • Mice (see )
  • Appropriate diet
  • Housing cages (individual housing, recommended)
  • Scale with 0.1 g accuracy (to weigh mice and food)
  • Container to accommodate mice during weighing
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Figures

  •   FigureFigure 29.B0.1 Example of a food trough for powdered diet. Powdered diet is placed in the barrel‐shaped container and the metal grid (which limits food spoiling and contamination) is positioned on top of the diet and secured with screws and a grid holder. Mice access food via the small holes of the grid.
  •   FigureFigure 29.B0.2 Effects of chow and high‐fat diets on body weight. Three groups of 7‐week male and three groups of 7‐week female C57BL/6J mice were housed at five animals per cage and fed ad libitum with chow diet (D04, Safe Diets), high‐fat/high‐sucrose diet (40% of energy as fat and 40% as sucrose; D12327, Research Diets), or very‐high‐fat diet (60% energy as fat; D12492, Research Diets). Body weight was recorded twice weekly over twenty weeks on ten animals per group.
  •   FigureFigure 29.B0.3 Decisional flowchart for test compound incorporation in a diet.

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Literature Cited

Literature Cited
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