Dietary Manipulation of Mouse Metabolism

Jérôme N. Feige1, Marie Lagouge1, Johan Auwerx2

1 Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France, 2 Ecole Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
Publication Name:  Current Protocols in Molecular Biology
Unit Number:  Unit 29B.5
DOI:  10.1002/0471142727.mb29b05s84
Online Posting Date:  October, 2008
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The maintenance of metabolic homeostasis relies on the balanced intake of nutrients from food. Consequently, diet composition strongly impacts whole‐body physiology. Dietary formulations with strong nutrient imbalances can lead to metabolic disorders, with lipids and simple sugars playing a prominent role. This unit describes how diet formulation can be modified to generate mouse models of human metabolic pathologies, and it details methodological procedures linked to dietary manipulations, including caloric restriction and introduction of a test compound. Curr. Protoc. Mol. Biol. 84:29B.5.1‐29B.5.12. © 2008 by John Wiley & Sons, Inc.

Keywords: metabolism; diet; mouse; diabetes; obesity

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Table of Contents

  • Introduction
  • Basic Protocol 1: Induction of Metabolic Pathologies Via Dietary Manipulation
  • Basic Protocol 2: Administration of a Test Compound Through the Diet
  • Basic Protocol 3: Caloric Restriction
  • Commentary
  • Literature Cited
  • Figures
  • Tables
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Basic Protocol 1: Induction of Metabolic Pathologies Via Dietary Manipulation

  • Appropriate mouse strain (e.g., C57BL/6 or see )
  • Mouse diet (standard or high‐fat diet) in powdered form
  • Test compound
  • Vehicle to dissolve compound (if required)
  • Group housing cages for mice
  • Scale with 0.1 g accuracy (to weigh food and mice)
  • Container to accommodate mice during weighing
  • Scale with 0.1 mg accuracy (to weigh compound)
  • Mortar and pestle for grinding the compound (if required)
  • Large container for mixing diet and test compound or automated mixing machine
  • Commercial pelleting machine (optional)
  • Desiccator (optional)

Basic Protocol 2: Administration of a Test Compound Through the Diet

  • Mice (see )
  • Appropriate diet
  • Housing cages (individual housing, recommended)
  • Scale with 0.1 g accuracy (to weigh mice and food)
  • Container to accommodate mice during weighing
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Literature Cited

Literature Cited
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