This unit describes sample preparation and sectioning methods for frozen tissue. Sections of this type are used in a variety of light microscopic procedures including in situ hybridization, immunohistochemistry, and enzyme histochemistry.
Basic Protocol 1: Specimen Preparation and Sectioning
Support Protocol 1: Fixation of Cryosections for In Situ Hybridization
Support Protocol 2: Tissue Fixation and Sucrose Infusion
Figure 14.2.1 Sectioning procedure. A to E illustrate cryosectioning and correspond to steps to of basic protocol; C to E also illustrate paraffin wax sectioning and correspond to steps to of support protocol, UNIT.
|Hollands, B. 1962. Histochemistry and microtomy of fresh‐frozen tissue. In Progress in Medical Laboratory Technique (F.J. Baker, ed.) pp. 112‐135. Butterworth, London|
|These references describe the basic cryosectioning method and applications in clinical pathology.|
|Zugibe, F. 1970. Diagnostic Histochemistry. C.V. Mosby, St. Louis.|