Separation Index: An Easy‐to‐Use Metric for Evaluation of Different Configurations on the Same Flow Cytometer

Martin Bigos1

1 Gladstone Institute of Virology and Immunology, San Francisco, California
Publication Name:  Current Protocols in Cytometry
Unit Number:  Unit 1.21
DOI:  10.1002/0471142956.cy0121s40
Online Posting Date:  April, 2007
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Abstract

A quick and understandable method, using material readily available in a biology laboratory, for evaluating the measurement sensitivity of flow cytometers is presented in detail. Two examples of its use, in determining an optimal emission filter and in evaluating compensation, are also presented.

Keywords: configuration; filters; sensitivity; evaluation

     
 
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Table of Contents

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Materials

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