
Current Protocols in Stem Cell Biology
Overview
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What's New in Current Protocols in Stem Cell
Biology
Supplement 19, December 2011
Unit 1B.9 Quantification of Pluripotency Transcription
Factor Levels in Embryonic Stem Cells by Flow Cytometry
Abstract | Full Text:
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Unit 1C.12 Live-Cell Immunofluorescence Staining of Human
Pluripotent Stem Cells
Abstract | Full Text:
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Unit 1H.4 Isolation and Culture of Adult Ciliary Epithelial
Cells, Previously Identified as Retinal Stem Cells, and Retinal
Progenitor Cells
Abstract | Full Text:
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Unit 3.5 Organotypic Explant Culture of Glioblastoma
Multiforme and Subsequent Single-Cell Suspension
Abstract | Full Text:
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Published in affiliation with the International Society for Stem Cell Research (ISSCR), Current Protocols in Stem Cell Biology (CPSC) covers the most fundamental protocols and methods in the rapidly growing field of stem cell biology.
Updated monthly, CPSC will constantly evolve with thelatest developments and breakthroughs in the field. Drawing on the expertise of leading researchers from around the world, Current Protocols in Stem Cell Biology includes methods and insights that will enhance the progress of global research.
To start, Current Protocols in Stem Cell Biology will cover research in:
- Embryonic Stem Cells - methods for isolation of stem cells from a variety of model organisms and humans, characterization of these cells and the undifferentiated state, induction of differentiation into cells of the mesodermal, endodermal, ectodermal, and extraembryonic lineages, and molecular and functional characterization of the differentiated state.
- Adult Stem Cells - isolation of progenitor stem cells from differentiated tissues, their characterization, and differentiation.
- Genetic Manipulation of Stem Cells - tools for manipulating the genetic content of stem cells and for marking stem cells.
Current Protocols in Stem Cell Biology will add new methods and ideas as the field expands. It employs the standardized presentation and format that has made Current Protocols the most respected source of methods for twenty years.
Edited by: Roger Patient (University of Oxford); Thorsten Schlaeger (Children's Hospital Boston); Evan Y. Snyder (Burnham Institute for Medical Research); Yukiko Yamashita (University of Michigan). Past Editors: Mick Bhatia (McMaster University); Susan J. Fisher (University of California San Francisco)
Developmental Editor: Karen Chambers
While the authors, editors, and publisher believe that the specification and usage of reagents, equipment, and devices, as set forth in this book, are in accord with current recommendations and practice at the time of publication, they accept no legal responsibility for any errors or omissions, and make no warranty,express or implied, with respect to material contained herein. In view of ongoing research, equipment modifications, changes in governmental regulations, and the constant flow of information relating to the use of experimental reagents, equipment, and devices, the reader is urged to review and evaluate the information provided in the package insert or instructions for each chemical, piece of equipment, reagent, or device for, among other things, any changes in the instructions or indication of usage and for added warnings and precautions. This is particularly important in regard to new or infrequently employed chemicals or experimental reagents. Moreover, the information presented herein is not a substitute for professional judgment, especially as concerns any applications in a clinical setting or the interpretation of results thereby obtained.



