Current Protocols in Stem Cell Biology
Last Update: February 09, 2012
Print ISSN: 1941-7322
Online ISSN: 1938-8969
- Overview
- Table of Contents
- New Protocols
- Sample Unit
- Editors & Contributors
Table of Contents
- Foreword
- Preface
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Chapter 1
Embryonic and Extraembryonic Stem Cells
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Section A
Isolation of Embryonic Stem Cells
- Isolation of Embryonic Stem Cells
- Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
- Unit 1A.2 Derivation of hESC from Intact Blastocysts
- Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
- Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
- Unit 1A.5 Generation of Human Embryonic Stem Cells
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Section B
Characterization of Embryonic Stem Cells
- Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
- Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High‐Throughput Microarray Platform
- Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
- Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
- Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
- Unit 1B.6 Characterization of X‐Chromosome Inactivation Status in Human Pluripotent Stem Cells
- Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
- Unit 1B.8 Identifying Translationally Regulated Genes During Stem Cell Differentiation
- Unit 1B.9 Quantification of Pluripotency Transcription Factor Levels in Embryonic Stem Cells by Flow Cytometry
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Section C
Culture and Maintenance of Undifferentiated Embryonic Stem Cells
- Culture and Maintenance of Undifferentiated Embryonic Stem Cells
- Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
- Unit 1C.2 Defined, Feeder‐Independent Medium for Human Embryonic Stem Cell Culture
- Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
- Unit 1C.4 Culture of Mouse Embryonic Stem Cells
- Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
- Unit 1C.6 Isolation of Human Placental Fibroblasts
- Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
- Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
- Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
- Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
- Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
- Unit 1C.12 Live‐Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
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Section D
Germ Layer Induction/Differentiation of Embryonic Stem Cells
- Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
- Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
- Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
- Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
- Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
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Section E
Extraembryonic Lineages
- Isolation of Stem Cells from Extraembryonic Tissues
- Unit 1E.1 Isolation of Human Placenta‐Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte‐Like Cells
- Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
- Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
- Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
- Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
- Unit 1E.6 Amnion Epithelial Cell Isolation and Characterization for Clinical Use
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Section F
Mesodermal Lineages
- Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
- Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum‐Free Medium
- Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
- Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
- Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
- Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
- Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors
- Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
- Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
- Unit 1F.10 Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac Progenitor Cells
- Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the Hanging‐Drop and Mass Culture Methods
- Unit 1F.12 Lentiviral Transduction and Clonal Selection of hESCs with Endothelial‐Specific Transgenic Reporters
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Section G
Endodermal Lineages
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Section H
Ectodermal Lineages
- Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
- Unit 1H.2 Time‐Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by Two‐Photon Microscopy
- Unit 1H.3 Basic Techniques in Human Mesenchymal Stem Cell Cultures: Differentiation into Osteogenic and Adipogenic Lineages, Genetic Perturbations, and Phenotypic Analyses
- Unit 1H.4 Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells
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Chapter 2
Somatic Stem Cells
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Section A
Hematopoietic Stem Cells
- Hematopoietic Stem Cells
- Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll‐Paque Density Gradient
- Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
- Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
- Unit 2A.4 Isolation and Assessment of Long‐Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
- Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
- Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues
- Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
- Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
- Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
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Section B
Non‐Hematopoietic Bone Marrow‐Derived Stem Cells
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Section C
Cardiovascular Stem Cells
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Section D
Neural Stem Cells
- Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
- Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
- Unit 2D.3 Long‐Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
- Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage Progression, and Maturation
- Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic Neurons from Rodent Brains
- Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere Technique
- Unit 2D.7 Neural Stem Cell Grafting in an Animal Model of Chronic Temporal Lobe Epilepsy
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Section E
Germline Stem Cells
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Section F
Gut Stem Cells
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Section G
Lung Stem Cells
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Chapter 3
Cancer Stem Cells
- Unit 3.1 Colon Cancer Stem Cells
- Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
- Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
- Unit 3.4 Isolation and Expansion of Regionally Defined Human Glioblastoma Cells In Vitro
- Unit 3.5 Organotypic Explant Culture of Glioblastoma Multiforme and Subsequent Single‐Cell Suspension
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Chapter 4
Manipulation of Potency
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Section A
iPS Cells
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Section B
Nuclear Transfer
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Chapter 5
Genetic Manipulation of Stem Cells
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Section A
Lineage Tracers in Stem Cells
- Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
- Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre‐Mediated Lineage Tracing
- Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
- Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
- Unit 5A.5 Kaede‐Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian Neocortical Neural Progenitors
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Section B
Homologous Recombination in Stem Cells
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Section C
RNA Interference in Stem Cells
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Appendix 1
Useful Information
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Appendix 2
Laboratory Equipment
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Appendix
Suppliers
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